4-Hydroxyethyl Piperazine Ethane Sulfonic Acid(HEPES)-CAS 7365-45-9
Product Name: 4-Hydroxyethyl Piperazine Ethane Sulfonic Acid
Abbreviation: HEPES
Chemical Name: 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic Acid
CAS No.: 7365-45-9
Grade: Biological Grade / Cosmetic Grade / Laboratory Reagent Grade
Purity: ≥99.0%
Appearance: White Crystalline Powder
Molecular Weight: 238.30
Molecular Formula: C₈H₁₈N₂O₄S
MOQ (Minimum Order Quantity): 1KG
Solubility: Easily soluble in water, slightly soluble in organic solvents, fast dissolution, clear and transparent solution
Buffering pH Range: 6.8 - 8.2 (Optimal biological activity pH interval)
pKa Value (25℃): 7.55
Key Characteristics: Ultra-stable pH buffering performance, low toxicity and high safety, light and heat stability, no interference with biological reactions, excellent system compatibility, long-term stable buffering effect
Storage Condition: Sealed, dry, cool and dark environment, avoid high temperature, humid environment and strong light irradiation, prevent agglomeration and deterioration
Shelf Life: 24 Months
Application: Widely used in cell culture buffer, biological experiment detection, cosmetic pH stabilization, biochemical reagent preparation, medical and biological engineering fields
HEPES, 4-Hydroxyethyl Piperazine Ethane Sulfonic Acid, Biological Buffer Agent, pH Stabilizer, Cell Culture Buffer, Low Toxicity Buffer Powder, Cosmetic pH Regulator, Laboratory Reagent
4-Hydroxyethyl Piperazine Ethane Sulfonic Acid(HEPES)-CAS 7365-45-9
High-purity HEPES (4-Hydroxyethyl Piperazine Ethane Sulfonic Acid) is a classic and efficient zwitterionic biological buffer raw material, widely recognized and applied in global biochemistry, life science and cosmetic industry. Different from traditional phosphate and citrate buffers, HEPES has an ideal pKa value close to physiological pH, providing stable and lasting pH buffering capacity in the neutral and weakly alkaline range.
It features extremely low biological toxicity, no cell irritation, no interference with enzyme activity, cell metabolism and biochemical reaction processes, and can maintain a stable pH environment for a long time even in open system culture.
The product has excellent light and thermal stability, will not decompose or fail under conventional experimental and production conditions, and will not produce harmful by-products.
In cosmetic formulas, it can efficiently stabilize the system pH value, avoid formula pH drift and deterioration caused by temperature change and long-term storage, improve product stability and skin compatibility. With high purity, low impurity and strong practicability, it is a universal core buffer raw material for biological research, medical detection and high-stability cosmetic formulas.
Key Benefits
• Ultra-Stable pH Buffering: Precise buffering range of 6.8-8.2, perfectly fit physiological and cosmetic formula pH environment, effectively prevent pH value drift and system instability.
• Low Toxicity & High Biosafety: Extremely low biological toxicity, non-irritating to cells and skin, no interference with biochemical reactions, safe for biological culture and long-term skin contact.
• Excellent Environmental Stability: Good light and heat resistance, stable performance in open system, not easy to decompose and fail, lasting buffering effect.
• No By-Product Pollution: No harmful decomposition by-products during use, no impact on experimental results and cosmetic formula purity, high purity and high stability.
• Fast Dissolution & Easy Operation: Fine crystalline powder, fast water dissolution, clear solution, no precipitation and impurity residue, convenient for experimental and industrial production.
• Wide Compatibility: Compatible with various biological reagents, culture media and cosmetic raw materials, no antagonistic reaction, strong formula adaptability.
Applications
• Biological Cell Culture: Core buffer for mammalian cell, plant cell and microbial culture, stabilize medium pH, ensure normal cell metabolism and growth, improve culture success rate.
• Biochemical Experiment & Detection: Used for enzyme reaction buffer, protein electrophoresis, immune detection, molecular biology experiments, ensure accurate and stable experimental data.
• High-Stability Cosmetics: Applied in skin care lotion, essence, facial mask and mild functional cosmetics, stabilize formula pH, improve product shelf stability and skin comfort.
• Medical & Biochemical Reagents: Preparation of in vitro diagnostic reagents, biological detection kits, standard buffer solutions for medical testing, high precision and strong stability.
• Biological Engineering Research: Widely used in biological fermentation, tissue engineering, drug screening and other scientific research and industrial production fields.
Certificate Of Analysis (COA)
Test Item | Standard Specification | Test Result |
Product Name | 4-Hydroxyethyl Piperazine Ethane Sulfonic Acid (HEPES) | Compliant |
CAS No. | 7365-45-9 | Compliant |
Appearance | White Crystalline Powder | Compliant |
Purity | ≥99.0% | 99.72% |
pKa Value (25℃) | 7.50 - 7.60 | 7.55 |
Loss on Drying | ≤0.3% | 0.11% |
Residue on Ignition | ≤0.1% | 0.05% |
Water Insoluble Matter | ≤0.01% | 0.005% |
Heavy Metal (Pb) | ≤10ppm | Qualified |
Microorganism | Sterile, Low Biotoxicity Standard | Qualified |
Conclusion | Conforms to biological & cosmetic grade quality standard | Qualified |
Frequently Asked Questions (FAQ)
• Q1: What is the MOQ of HEPES?
A1: The standard MOQ is 1KG, and sample orders are supported for quality testing.
• Q2: What certification documents can be provided?
A2: Complete COA, MSDS and full set of global customs clearance documents are available.
• Q3: What is the recommended dosage for cosmetic formulas?
A3: Conventional addition ratio is 0.1%-0.5%, which can stably lock formula pH and improve product stability.
• Q4: What is the difference between HEPES and traditional buffers?
A4: HEPES has lower toxicity, stronger environmental stability, no interference with biological reactions, and stable buffering effect in open systems, far superior to phosphate and citrate buffers.
• Q5: Is HEPES suitable for long-term cell culture?
A5: Yes, low biotoxicity and stable pH performance, very suitable for long-term cell culture and high-precision biochemical experiments.
• Q6: Do you support stable large-batch supply all year round?
A6: Sufficient factory capacity ensures continuous and stable bulk supply throughout the year.
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